Human Hepatic Stellate Cells

HLSC

SPECIES:   Human

TISSUE:   Liver

CELL TYPE:   Stellate cells

FORMAT:   Cryopreserved following initial culture

Human Hepatic Stellate Cells (HSC) from Samsara are isolated from viable human liver by differential centrifugation and further purified using cell culture conditions. Cells are cultured immediately after isolation then cryopreserved at passage 0. Standard product characterization includes lot-specific post-thaw yield and viability as well as expression of Desmin, GFAP, α-SMA, TE-7 and CD31 by immunocytochemistry (ICC). Comprehensive donor history report includes medical history and board-certified pathologist assessment of NAFLD activity score (NAS) and Batts-Ludwig fibrosis score. All livers come from consented donors and test negative for HIV I/II, HBV, HCV and Syphilis prior to acceptance.

PASSAGE NUMBER:   p0

VOLUME:   1.0 mL per vial

POST-THAW YIELD:   > 0.5 x 106 cells per vial

CELL SIZE RANGE:   12 – 29 microns

DONOR AVAILABILITY:   Healthy or diseased, single donor

Cell viability and yield determined by image cytometry AO/PI dual staining

Post-thaw plating to confirm characteristic morphology

Purity assessed by utilizing ICC for expression of Desmin, GFAP, α-SMA, TE-7, CD31

SHIPPING CONDITION:   Dry ice, Liquid nitrogen dry vapor shipper upon request

SHORT-TERM STORAGE (< 3 DAYS):   -80°C

LONG-TERM STORAGE (> 3 DAYS):   Liquid nitrogen vapor phase

Complete the online Quote Request Form and a Samsara team member will contact you within 24 hours to determine which donor and format options are best suited for your research needs.