Human Kupffer Cells
CELL TYPE: Kupffer cells
FORMAT: Cryopreserved following isolation
Human Kupffer Cells (KC) from Samsara are isolated from viable human liver and cryopreserved immediately after purification by immunoselection. Isolated KCs mount a cytokine response to pro-inflammatory stimulus, lipopolysaccharide (LPS). Standard product characterization includes lot-specific post-thaw yield and viability as well as CD11b and CD68 biomarker expression. Comprehensive donor history report includes medical history and board-certified pathologist assessment of NAFLD activity score (NAS) and Batts-Ludwig fibrosis score. All livers come from consented donors and test negative for HIV I/II, HBV, HCV and Syphilis prior to acceptance.
VOLUME: 1.0 mL per vial
POST-THAW YIELD: > 0.5 x 106 cells per vial
CELL SIZE RANGE: 8 – 15 microns
DONOR AVAILABILITY: Healthy or diseased, single donor
Cell viability and yield determined by image cytometry AO/PI dual staining
Post-thaw plating to confirm characteristic morphology
Cell functionality measured by cytokine response to LPS stimulation
Purity assessed by expression of CD11b and CD68
SHIPPING CONDITION: Dry ice, Liquid nitrogen dry vapor shipper upon request
SHORT-TERM STORAGE (< 3 DAYS): -80°C
LONG-TERM STORAGE (> 3 DAYS): Liquid nitrogen vapor phase
Complete the online Quote Request Form and a Samsara team member will contact you within 24 hours to determine which donor and format options are best suited for your research needs.